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Forschungsdatenbank PMU-SQQUID

Human Cerebrospinal Fluid Promotes Neuronal Viability and Activity of Hippocampal Neuronal Circuits In Vitro
Perez-Alcazar, M; Culley, G; Lyckenvik, T; Mobarrez, K; Bjorefeldt, A; Wasling, P; Seth, H; Asztely, F; Harrer, A; Iglseder, B; Aigner, L; Hanse, E; Illes, S
FRONT CELL NEUROSCI. 2016; 10:
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Aigner Ludwig
Harrer Andrea
Iglseder Bernhard
Illes Sebastian

Abstract

For decades it has been hypothesized that molecules within the cerebrospinal fluid (CSF) diffuse into the brain parenchyma and influence the function of neurons. However, the functional consequences of CSF on neuronal circuits are largely unexplored and unknown. A major reason for this is the absence of appropriate neuronal in vitro model systems, and it is uncertain if neurons cultured in pure CSF survive and preserve electrophysiological functionality in vitro. In this article, we present an approach to address how human CSF (hCSF) influences neuronal circuits in vitro. We validate our approach by comparing the morphology, viability, and electrophysiological function of single neurons and at the network level in rat organotypic slice and primary neuronal cultures cultivated either in hCSF or in defined standard culture media. Our results demonstrate that rodent hippocampal slices and primary neurons cultured in hCSF maintain neuronal morphology and preserve synaptic transmission. Importantly, we show that hCSF increases neuronal viability and the number of electrophysiologically active neurons in comparison to the culture media. In summary, our data indicate that hCSF represents a physiological environment for neurons in vitro and a superior culture condition compared to the defined standard media. Moreover, this experimental approach paves the way to assess the functional consequences of CSF on neuronal circuits as well as suggesting a novel strategy for central nervous system (CNS) disease modeling.


Find related publications in this database (Keywords)

human cerebrospinal fluid
hippocampal neuronal function
hippocampal neuronal survival
organotypic hippocampal slice cultures
hippocampal neuronal cultures
multi-electrode array technology