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Forschungsdatenbank PMU-SQQUID

The Repair of Critical-Sized Bone Defects Using Expedited, Autologous BMP-2 Gene-Activated Fat Implants
Betz, OB; Betz, VM; Abdulazim, A; Penzkofer, R; Schmitt, B; Schroder, C; Mayer-Wagner, S; Augat, P; Jansson, V; Muller, PE
TISSUE ENG PT A. 2010; 16(3): 1093-1101.
Originalarbeiten (Zeitschrift)


Augat Peter


The repair of bone defects can be induced experimentally with bone morphogenetic protein-2 (BMP-2) producing fat-derived stem cells, but this ex vivo tissue engineering method requires the isolation and long-term culture of autologous cells. To develop an expedited bone repair strategy, we transferred BMP-2 cDNA directly to autologous fat tissue fragments that were held in culture for only 24 h before implantation. We evaluated the ability of such gene-activated fat grafts to regenerate large segmental bone defects in rats. Fat tissue was harvested from 2 of 35 male Fischer 344 rats used for this study. The fat tissue fragments were incubated with an adenoviral vector carrying the cDNA encoding either BMP-2 or green florescent protein (GFP), or they remained unmodified. According to their group, the segmental femoral bone defects of 33 rats were filled press fit with either BMP-2-activated fat tissue, GFP-transduced fat tissue, or unmodified fat tissue. Another control group remained untreated. Femora were evaluated by radiographs, microcomputed tomography, biomechanical torsional testing, and histology. Radiographically and histologically, 100% of the femora treated with BMP-2activated fat grafts were bridged at 6 weeks after surgery. The femora of this group exceeded the bone volume and the biomechanical stability of intact, contralateral femora. Control defects receiving no treatment, unmodified fat tissue, or GFP-transduced fat were filled with fibrous or adipose tissue, as evaluated by histology. The use of BMP-2 gene-activated fat tissue grafts represents an expedited and effective bone repair strategy that does not require the extraction and expansion of stem cells.

Useful keywords (using NLM MeSH Indexing)

Adipose Tissue/metabolism*

Adipose Tissue/transplantation



Bone Morphogenetic Protein 2/genetics*

Bone Morphogenetic Protein 2/metabolism

Enzyme-Linked Immunosorbent Assay



Green Fluorescent Proteins/metabolism

Implants, Experimental*


Organ Size


Rats, Inbred F344


Transcriptional Activation*

Transduction, Genetic

Wound Healing

X-Ray Microtomography