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Forschungsdatenbank PMU-SQQUID

Effects of crystalline glucocorticoid triamcinolone acetonide on cultered human supraspinatus tendon cells.
Tempfer, H; Gehwolf, R; Lehner, C; Wagner, A; Mtsariashvili, M; Bauer, HC; Resch, H; Tauber, M;
ACTA ORTHOP. 2009; 80(3): 357-362.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Bauer Hans Christian
Gehwolf Renate
Lehner Christine
Resch Herbert
Tauber Mark
Tempfer Herbert
Wagner Andrea

Abstract

BACKGROUND
Rotator cuff tears are a common cause of shoulder pain and impairment. Subacromial glucocorticoid injections are widely used for treatment of epiphenomenons of chronic impingement syndrome with the possible side effects of tendon rupture and impaired tendon healing.
Using qRT-PCR, western blot, immunoflourescence, and measurement of 3H-thymidine uptake we investigated the effects of the crystalline glucocorticoid triamcinolone acetonide (TAA) when added to the culture medium of isolated human rotator cuff tendon cells.
After 2 weeks of incubation, the cells had lost their fibroblastic appearance and parallel orientation, which is characteristic of cellular degeneration in vivo. Moreover, expression and secretion of collagen I was strongly reduced, and there was a decrease in proliferation rate. Cell migration was blocked and the rate of expression of the matrix metalloproteinases MMP2, MMP8, MMP9, and MMP13 was reduced, but expression of TIMP1 (a tissue inhibitor of MMPs) was upregulated, indicating a reduction in the cellular capacity for tendon repair. In addition, changes in cellular differentiation were observed: the number of adipocytes increased and levels of the protein Sox9-a marker of differentiating and mature chondrocytes-were elevated in triamcinolone acetonide treated cells.
These results may indicate that the use of TAA is one reason for weaker mechanical tendon properties and for the high rate of re-rupture after supraspinatus tendon repair.


Useful keywords (using NLM MeSH Indexing)

Adolescent

Adult

Anti-Inflammatory Agents/administration*

dosage

Anti-Inflammatory Agents/adverse effects

Anti-Inflammatory Agents/pharmacology*

Cell Proliferation/drug effects

Cells, Cultured

Collagen/biosynthesis

Collagen/secretion

Female

Glucocorticoids/administration*

dosage

Glucocorticoids/adverse effects

Glucocorticoids/pharmacology*

Humans

Male

Matrix Metalloproteinases/metabolism

Middle Aged

Rotator Cuff/cytology

Rotator Cuff/drug effects

Rotator Cuff/metabolism

Tendons/cytology

Tendons/drug effects*

Tendons/metabolism

Triamcinolone/administration*

dosage

Triamcinolone/adverse effects

Triamcinolone/pharmacology*

Young Adult