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Forschungsdatenbank PMU-SQQUID

DNA immunization in vivo down-regulates nuclear all-trans retinoic acid receptors in mouse spleen cells.
Brtko, J; Hartl, A; Weiss, R; Bernhaupt, A; Scheiblhofer, S; Mostböck, S; Thalhamer, J;
Mol Cell Endocrinol. 2000; 165(1-2):107-113
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Hartl Arnulf Josef

Abstract


Nuclear retinoid receptors - retinoic acid inducible transcription factors - participate in pathways influencing many components of the immune system. In the present study in vivo effects of DNA-based immunization of mice on binding parameters of all-trans retinoic acid receptors (RARs) in spleen cell nuclei was investigated. A eucaryotic expression vector encoding the gene for the model enzyme beta-galactosidase of Escherichia coli (pCMV-beta) was used for intradermal injection. Furthermore, immunostimulatory CpG motifs, which stimulate the expression of various cytokines and may serve as a "danger signal" for the mammalian immune system, were coinjected as oligodeoxynucleotides. The results demonstrate that the concentration of RARs was significantly reduced in the late phase of the primary immune response (21 days after injection of plasmid DNA-indicated by high affinity IgG antibodies and IFN-gamma expression). Coinjection of CpG motifs did not change the course of the humoral response but enhanced and accelerated the proliferative response and expression of IFN-gamma, which correlated with the reduced RARs concentration.


Useful keywords (using NLM MeSH Indexing)

Animals

Base Sequence

Cell Nucleus/metabolism

CpG Islands/genetics

CpG Islands/immunology

Down-Regulation

Escherichia coli/enzymology

Escherichia coli/genetics

Female

Immunization

Lac Operon

Mice

Mice, Inbred BALB C

Oligodeoxyribonucleotides/genetics

Receptors, Retinoic Acid/metabolism*

Spleen/immunology*

Spleen/metabolism*

Vaccines, DNA/genetics

Vaccines, DNA/pharmacology*

beta-Galactosidase/genetics


Find related publications in this database (Keywords)

nuclear retinoic acid receptor
DNA immunization
beta-galactosidase
immune response
CpG motifs
BALB/c mice