PMU-Autor/inn/enHartl Arnulf Josef
The present study outlines the characterization of a DNA-based immune response against the OspC antigen, one of the most promising candidates for a Borrelia vaccine. Balb/c mice were injected intradermally with plasmid DNA encoding the OspC gene (lacking the natural leader sequence) under transcriptional control of the cytomegalovirus (CMV) promotor. Immunization with this construct elicited only a marginal response, which was drastically improved by a fusion construct containing the human tissue plasminogen activator (hTPA) signal sequence. The results indicate that for DNA-based immunization against OspC an ER-targeting signal may be necessary for both antibody production as well as cellular immune responses.
Useful keywords (using NLM MeSH Indexing)
Amino Acid Sequence
Bacterial Outer Membrane Proteins/genetics*
Molecular Sequence Data
Protein Sorting Signals/immunology*
Find related publications in this database (Keywords)DNA-based immunization