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Forschungsdatenbank PMU-SQQUID

Improvement of the immune response against plasmid DNA encoding OspC of Borrelia by an ER-targeting leader sequence.
Weiss, R; Dürnberger, J; Mostböck, S; Scheiblhofer, S; Hartl, A; Breitenbach, M; Strasser, P; Dorner, F; Livey, I; Crowe, B; Thalhamer, J;
Vaccine. 1999; 18(9-10):815-824
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Hartl Arnulf Josef
Strasser Peter

Abstract


The present study outlines the characterization of a DNA-based immune response against the OspC antigen, one of the most promising candidates for a Borrelia vaccine. Balb/c mice were injected intradermally with plasmid DNA encoding the OspC gene (lacking the natural leader sequence) under transcriptional control of the cytomegalovirus (CMV) promotor. Immunization with this construct elicited only a marginal response, which was drastically improved by a fusion construct containing the human tissue plasminogen activator (hTPA) signal sequence. The results indicate that for DNA-based immunization against OspC an ER-targeting signal may be necessary for both antibody production as well as cellular immune responses.


Useful keywords (using NLM MeSH Indexing)

Amino Acid Sequence

Animals

Antibodies, Bacterial/biosynthesis*

Antigens, Bacterial*

Bacterial Outer Membrane Proteins/genetics*

Base Sequence

Borrelia/genetics

Borrelia/immunology*

DNA, Bacterial/immunology*

Endoplasmic Reticulum/metabolism

Humans

Immunity, Cellular

Immunoglobulin G/biosynthesis

Interferon-gamma/biosynthesis

Interleukin-4/biosynthesis

Mice

Molecular Sequence Data

Protein Sorting Signals/immunology*

Spleen/immunology

Spleen/metabolism


Find related publications in this database (Keywords)

DNA-based immunization
OspC
borreliosis