Forschungsdatenbank PMU-SQQUID

PMU-Autor/inn/en

Abstract

Inflammatory cytokines in vitro are believed to be involved in the regulation of type I iodothyronine 5"-deiodinase (5"-DI) activity. The present study was undertaken to investigate in vivo effects of DNA immunization of mice on the 5"-DI activity in the liver. A mammalian expression vector encoding the beta-galactosidase (pCMV-betagal) was used for intradermal immunization. Furthermore, immunostimulatory CpG motifs, which induce the expression of IL-6, IL-12, IL-18, TNF-alpha/beta and IFN-gamma were coinjected as oligodeoxynucleotides. From our data we conclude that the activity of 5"-DI in mouse liver when compared to non-immunized animals (100%) was found to be significantly enhanced by DNA immunization 2 weeks (175.7%) or 3 weeks (192.6%) after the plasmid injection. In addition, the activity of the 5"-DI in mouse liver was markedly enhanced 2 weeks (252.4%) or 3 weeks (243.3%) after the injection when CpG motifs were applied together with the plasmid DNA.

Useful keywords (using NLM MeSH Indexing)

Animals

Cytokines/biosynthesis

Cytokines/immunology

DNA/administration*

dosage

DNA/immunology*

Female

Immunization

Inflammation/immunology

Iodide Peroxidase/biosynthesis

Iodide Peroxidase/immunology*

Liver/enzymology

Liver/immunology*

Mice

Mice, Inbred BALB C

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