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Forschungsdatenbank PMU-SQQUID

Retinoic acid receptor status in mouse spleen during a primary immune response against beta-galactosidase.
Brtko, J; Hartl, A; Weiss, R; Scheiblhofer, S; Mostboeck, S; Thalhamer, J;
Endocr Regul. 2000; 34(3):113-118
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Hartl Arnulf Josef

Abstract

OBJECTIVE
Evaluation of the dynamics of all-trans retinoic acid receptor binding properties in mouse spleen nuclear extracts during a primary immune response against beta-galactosidase.
Female BALB/c mice, aged between 5 and 6 weeks were immunized intradermally into the shaved back (4 spots each) with 100 microg beta-galactosidase in 100 microl sterile phosphate buffered saline (pH 7.2) and blood was taken by tail bleeding on days 0 (preimmune serum), 4 and 6. Production of antibody in serum and the detection of cytokines (IL-4, IFN-gamma) from proliferation supernatants were determined by ELISA. Antigen-specific proliferation assay of isolated spleen cells was based on [3H]-thymidine incorporation measured in a liquid scintillation counter. Both, the maximal binding capacity (Bmax) and the affinity (Ka) of all-trans retinoic acid nuclear receptors (RAR) were evaluated according to Brtko (1994).
Injection of beta-galactosidase induced the first detectable antibody responses on day 4 (IgM) and on day 6 (IgG). These points of time, reflecting the early and the mature immune response served to measure the antigen-specific proliferation and production of IL-4 and IFN-gamma in the supernatants of the proliferation cultures as well as all-trans retinoic acid receptor (RAR) binding characteristics in spleen nuclear proteins. The RAR Bmax was significantly (P<0.05) decreased only at the time of the first specific IgG antibody production.
The data obtained indicate the involvement of RAR in the late phase of an in vivo immune response.


Useful keywords (using NLM MeSH Indexing)

Animals

Antibody Formation*

Antigens/immunology

Antigens/metabolism

Cell Division

Enzyme-Linked Immunosorbent Assay

Female

Immunity, Cellular

Immunization

Immunoglobulin G/biosynthesis

Immunoglobulin M/biosynthesis

Interferon-gamma/biosynthesis

Interleukin-4/biosynthesis

Mice

Mice, Inbred BALB C

Receptors, Retinoic Acid/metabolism*

Spleen/immunology*

Spleen/metabolism*

Spleen/ultrastructure

Tretinoin/metabolism

beta-Galactosidase/immunology*