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Forschungsdatenbank PMU-SQQUID

Improvement of antisera raised against complex antigen mixtures by the use of heterologous sources of antigen for immunization.
Hammerl, P; Hartl, A; Thalhamer, J;
J Immunol Methods. 1993; 160(2):155-161
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Hartl Arnulf Josef

Abstract


Polyspecific antisera against antigen mixtures are important tools for many experimental purposes. However, following immunization with extracts containing a great number of different proteins, many antigens remain non-immunogenic. Therefore, the improvement of the antibody spectrum of antisera is an essential goal in maximising the power and applicability of many serological analyses. In the present report we demonstrate that the additional use of heterologous (i.e., antigenically related, but not identical) antigen mixtures for immunization increases the variety of antibodies in polyspecific antisera as well as the antibody titer against weak immunogens.


Useful keywords (using NLM MeSH Indexing)

Animals

Antibodies, Bacterial/immunology

Antibody Formation*

Antigens, Bacterial/administration*

dosage*

Bacterial Proteins/immunology

Blotting, Western

Escherichia coli/immunology*

Molecular Weight

Rabbits

Species Specificity


Find related publications in this database (Keywords)

ESCHERICHIA-COLI ANTIGEN
IMMUNIZATION
IMMUNE SUPPRESSION
ANTIGENIC COMPETITION
HETEROLOGOUS ANTISERUM
IMMUNOBLOTTING