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Forschungsdatenbank PMU-SQQUID

Halobacterium noricense sp. nov., an archaeal isolate from a bore core of an alpine Permian salt deposit, classification of Halobacterium sp. NRC-1 as a strain of H. salinarum and emended description of H. salinarum.
Gruber, C; Legat, A; Pfaffenhuemer, M; Radax, C; Weidler, G; Busse, HJ; Stan-Lotter, H;
Extremophiles. 2004; 8(6):431-439
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Weidler Gerhard

Abstract

Two rod-shaped haloarchaeal strains, A1 and A2, were isolated from a bore core from a salt mine in Austria. The deposition of the salt is thought to have occurred during the Permian period (225-280 million years ago). The 16S rDNA sequences of the strains were 97.1% similar to that of the type species of the genus Halobacterium, which was also determined in this work. Polar lipids consisted of C20-C20 derivatives of phosphatidylglycerol, methylated phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether and sulfated tetraglycosyl diether. Optimal salinity for growth was 15-17.5% NaCl; Mg++ was tolerated up to a concentration of 1 M. The DNA-DNA reassociation value of strain A1T was 25% with H. salinarum DSM 3754T and 41% with Halobacterium sp. NRC-1, respectively. Based on these results and other properties, e.g. whole cell protein patterns, menaquinone content and restriction patterns of DNA, strains A1 and A2 are members of a single species, for which we propose the name H. noricense. The type strain is A1 (DSM 15987T, ATCC BAA-852T, NCIMB 13967T). Since we present evidence that Halobacterium sp. NRC-1 is a member of H. salinarum, an emended description of H. salinarum is provided.


Useful keywords (using NLM MeSH Indexing)

Archaeal Proteins/isolation*

purification

Austria

Base Composition

Carotenoids/analysis

DNA, Archaeal/chemistry

DNA, Archaeal/genetics

DNA, Ribosomal/chemistry

DNA, Ribosomal/genetics

Genes, Archaeal

Geologic Sediments/microbiology*

Halobacterium/chemistry

Halobacterium/classification

Halobacterium/genetics

Halobacterium/isolation*

purification*

Halobacterium salinarum/classification*

Halobacterium salinarum/genetics

Lipids/analysis

Lipids/chemistry

Microscopy, Electron, Scanning

Phylogeny

Plasmids/genetics

RNA, Archaeal/genetics

RNA, Ribosomal, 16S/genetics

Sodium Chloride

Species Specificity

Terminology as Topic

Vitamin K 2/analysis


Find related publications in this database (Keywords)

Halobacterium noricense
Halobacterium salinarum
Halobacterium sp
NRC-1
Haloarchaea
salt mine
bore core
longevity