PMU-Autor/inn/en
Weidler GerhardAbstract
Two rod-shaped haloarchaeal strains, A1 and A2, were isolated from a bore core from a salt mine in Austria. The deposition of the salt is thought to have occurred during the Permian period (225-280 million years ago). The 16S rDNA sequences of the strains were 97.1% similar to that of the type species of the genus Halobacterium, which was also determined in this work. Polar lipids consisted of C20-C20 derivatives of phosphatidylglycerol, methylated phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether and sulfated tetraglycosyl diether. Optimal salinity for growth was 15-17.5% NaCl; Mg++ was tolerated up to a concentration of 1 M. The DNA-DNA reassociation value of strain A1T was 25% with H. salinarum DSM 3754T and 41% with Halobacterium sp. NRC-1, respectively. Based on these results and other properties, e.g. whole cell protein patterns, menaquinone content and restriction patterns of DNA, strains A1 and A2 are members of a single species, for which we propose the name H. noricense. The type strain is A1 (DSM 15987T, ATCC BAA-852T, NCIMB 13967T). Since we present evidence that Halobacterium sp. NRC-1 is a member of H. salinarum, an emended description of H. salinarum is provided.
Useful keywords (using NLM MeSH Indexing)
Archaeal Proteins/isolation*
purification
Austria
Base Composition
Carotenoids/analysis
DNA, Archaeal/chemistry
DNA, Archaeal/genetics
DNA, Ribosomal/chemistry
DNA, Ribosomal/genetics
Genes, Archaeal
Geologic Sediments/microbiology*
Halobacterium/chemistry
Halobacterium/classification
Halobacterium/genetics
Halobacterium/isolation*
purification*
Halobacterium salinarum/classification*
Halobacterium salinarum/genetics
Lipids/analysis
Lipids/chemistry
Microscopy, Electron, Scanning
Phylogeny
Plasmids/genetics
RNA, Archaeal/genetics
RNA, Ribosomal, 16S/genetics
Sodium Chloride
Species Specificity
Terminology as Topic
Vitamin K 2/analysis
Find related publications in this database (Keywords)
Halobacterium noricense