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Forschungsdatenbank PMU-SQQUID

MMP-9 haplotypes and carotid artery atherosclerosis: an association study introducing a novel multicolour multiplex RealTime PCR protocol.
Rauch, I; Iglseder, B; Paulweber, B; Ladurner, G; Strasser, P;
Eur J Clin Invest. 2008; 38(1):24-33
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Iglseder Bernhard
Paulweber Bernhard
Strasser Peter

Abstract

BACKGROUND
Among other matrix metalloproteinases (MMPs), gelatinase B (MMP-9) is discussed to be associated with the pathogenesis of vascular diseases. Two single nucleotide polymorphisms (SNPs) of the MMP-9 gene, C-1562T in the promoter region and a G/A transition in exon 6 (R + 279Q), have been addressed in previous association studies which, however, produced conflicting results.
A novel multiplex RealTime PCR protocol for the fast and simultaneous detection of both polymorphisms is presented, which was used for genotyping 1737 participants of a prospective study investigating genetic factors influencing the progression of atherosclerosis.
Haplotype analysis revealed -1562C/+279Q as the major haplotype in this population. Allelic distribution of the C-1562T polymorphism was consistent with data published for similar cohorts; however, we found that R + 279Q allelic distribution appears to vary significantly among Caucasian populations. Considering clinical data available from 1487 participants, we found significant associations between the presence of atherosclerotic plaque and the CA-haplotype in men (P = 0.028, phi = 0.08), and between the AG variant of exon 6 and common carotid artery intima-media thickness (CIMT) in women (P = 0.004, Eta(2) = 0.019).
In summary, our results demonstrate associations of MMP-9 genotypes with different stages of carotid atherosclerosis.


Useful keywords (using NLM MeSH Indexing)

Adult

Aged

Arteriosclerosis/diagnostic imaging

Arteriosclerosis/genetics*

Carotid Artery Diseases/genetics*

Female

Haplotypes/genetics

Humans

Male

Matrix Metalloproteinase 9/genetics*

Middle Aged

Polymerase Chain Reaction/methods

Polymorphism, Genetic/genetics*

Prospective Studies

Ultrasonography


Find related publications in this database (Keywords)

atherosclerosis
MMP-9
RealTime PCR
SNP