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Forschungsdatenbank PMU-SQQUID

Use of genetic profiling in leprosy to discriminate clinical forms of the disease.
Bleharski, JR; Li, H; Meinken, C; Graeber, TG; Ochoa, MT; Yamamura, M; Burdick, A; Sarno, EN; Wagner, M; Röllinghoff, M; Rea, TH; Colonna, M; Stenger, S; Bloom, BR; Eisenberg, D; Modlin, RL;
Science. 2003; 301(5639): 1527-1530.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Wagner Manfred

Abstract

Leprosy presents as a clinical and immunological spectrum of disease. With the use of gene expression profiling, we observed that a distinction in gene expression correlates with and accurately classifies the clinical form of the disease. Genes belonging to the leukocyte immunoglobulin-like receptor (LIR) family were significantly up-regulated in lesions of lepromatous patients suffering from the disseminated form of the infection. In functional studies, LIR-7 suppressed innate host defense mechanisms by shifting monocyte production from interleukin-12 toward interleukin-10 and by blocking antimicrobial activity triggered by Toll-like receptors. Gene expression profiles may be useful in defining clinical forms of disease and providing insights into the regulation of immune responses to pathogens.


Useful keywords (using NLM MeSH Indexing)

Algorithms

Cluster Analysis

Colony Count, Microbial

Cytokines/genetics

Cytokines/metabolism

Gene Expression Profiling*

Gene Expression Regulation*

Genes, Immunoglobulin

Humans

Immunity, Cellular

Immunity, Innate

Leprosy, Lepromatous/classification*

Leprosy, Lepromatous/genetics*

Leprosy, Lepromatous/immunology

Leprosy, Lepromatous/physiopathology

Leprosy, Tuberculoid/classification*

Leprosy, Tuberculoid/genetics*

Leprosy, Tuberculoid/immunology

Leprosy, Tuberculoid/physiopathology

Macrophages, Alveolar/microbiology

Membrane Glycoproteins/immunology

Mycobacterium tuberculosis/growth*

development

Mycobacterium tuberculosis/immunology

Oligonucleotide Array Sequence Analysis

Polymerase Chain Reaction

Principal Component Analysis

Receptors, Cell Surface/immunology

Receptors, Immunologic/genetics

Receptors, Immunologic/metabolism

Toll-Like Receptors

Up-Regulation