PMU-Autor/inn/en
Hainzl StefanAbstract
IL-31 is a T cell-derived cytokine that signals via a heterodimeric receptor composed of IL-31Rα and oncostatin M receptor β. Although several studies have aimed to investigate IL-31-mediated effects, the biological functions of this cytokine are currently not well understood. IL-31 expression correlates with the expression of IL-4 and IL-13 and is associated with atopic dermatitis in humans, indicating that IL-31 is involved in Th2-mediated skin inflammation. Because dendritic cells are the main activators of Th cell responses, we posed the question of whether dendritic cells express the IL-31R complex and govern immune responses triggered by IL-31. In the current study, we report that primary human CD1c(+) as well as monocyte-derived dendritic cells significantly upregulate the IL-31Rα receptor chain upon stimulation with IFN-γ. EMSAs, chromatin immunoprecipitation assays, and small interfering RNA-based silencing assays revealed that STAT1 is the main transcription factor involved in IFN-γ-dependent IL-31Rα expression. Subsequent IL-31 stimulation resulted in a dose-dependent release of proinflammatory mediators, including TNF-α, IL-6, CXCL8, CCL2, CCL5, and CCL22. Because these cytokines are crucially involved in skin inflammation, we hypothesize that IL-31-specific activation of dendritic cells may be part of a positive feedback loop driving the progression of inflammatory skin diseases.
Useful keywords (using NLM MeSH Indexing)
Cells, Cultured
Dendritic Cells/immunology*
Dendritic Cells/metabolism*
Dendritic Cells/pathology
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Humans
Inflammation/immunology
Inflammation/metabolism
Inflammation/pathology
Inflammation Mediators/metabolism*
Inflammation Mediators/physiology
Interferon-gamma/physiology*
Receptors, Interleukin/biosynthesis*
Receptors, Interleukin/genetics
Receptors, Interleukin/physiology
STAT1 Transcription Factor/physiology*
Skin Diseases/immunology
Skin Diseases/metabolism
Skin Diseases/pathology