' '
Deutsch | English    

Forschungsdatenbank PMU-SQQUID

Dendritic cells activated by IFN-γ/STAT1 express IL-31 receptor and release proinflammatory mediators upon IL-31 treatment.
Horejs-Hoeck, J; Schwarz, H; Lamprecht, S; Maier, E; Hainzl, S; Schmittner, M; Posselt, G; Stoecklinger, A; Hawranek, T; Duschl, A;
J IMMUNOL. 2012; 188(11): 5319-5326.
Originalarbeiten (Zeitschrift)


Hainzl Stefan
Hawranek Thomas


IL-31 is a T cell-derived cytokine that signals via a heterodimeric receptor composed of IL-31Rα and oncostatin M receptor β. Although several studies have aimed to investigate IL-31-mediated effects, the biological functions of this cytokine are currently not well understood. IL-31 expression correlates with the expression of IL-4 and IL-13 and is associated with atopic dermatitis in humans, indicating that IL-31 is involved in Th2-mediated skin inflammation. Because dendritic cells are the main activators of Th cell responses, we posed the question of whether dendritic cells express the IL-31R complex and govern immune responses triggered by IL-31. In the current study, we report that primary human CD1c(+) as well as monocyte-derived dendritic cells significantly upregulate the IL-31Rα receptor chain upon stimulation with IFN-γ. EMSAs, chromatin immunoprecipitation assays, and small interfering RNA-based silencing assays revealed that STAT1 is the main transcription factor involved in IFN-γ-dependent IL-31Rα expression. Subsequent IL-31 stimulation resulted in a dose-dependent release of proinflammatory mediators, including TNF-α, IL-6, CXCL8, CCL2, CCL5, and CCL22. Because these cytokines are crucially involved in skin inflammation, we hypothesize that IL-31-specific activation of dendritic cells may be part of a positive feedback loop driving the progression of inflammatory skin diseases.

Useful keywords (using NLM MeSH Indexing)

Cells, Cultured

Dendritic Cells/immunology*

Dendritic Cells/metabolism*

Dendritic Cells/pathology






Inflammation Mediators/metabolism*

Inflammation Mediators/physiology


Receptors, Interleukin/biosynthesis*

Receptors, Interleukin/genetics

Receptors, Interleukin/physiology

STAT1 Transcription Factor/physiology*

Skin Diseases/immunology

Skin Diseases/metabolism

Skin Diseases/pathology