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Forschungsdatenbank PMU-SQQUID

A new gene-finding tool: using the Caenorhabditis elegans operons for identifying functional partner proteins in human cells.
Eichmüller, S; Vezzoli, V; Bazzini, C; Ritter, M; Fürst, J; Jakab, M; Ravasio, A; Chwatal, S; Dossena, S; Bottà, G; Meyer, G; Maier, B; Valenti, G; Lang, F; Paulmichl, M;
J BIOL CHEM. 2004; 279(8): 7136-7146.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Dossena Silvia
Jakab Martin
Paulmichl Markus
Ritter Markus

Abstract

How can a large number of different phenotypes be generated by a limited number of genotypes? Promiscuity between different, structurally related and/or unrelated proteins seems to provide a plausible explanation to this pertinent question. Strategies able to predict such functional interrelations between different proteins are important to restrict the number of putative candidate proteins, which can then be subjected to time-consuming functional tests. Here we describe the use of the operon structure of the nematode genome to identify partner proteins in human cells. In this work we focus on ion channels proteins, which build an interface between the cell and the outside world and are responsible for a growing number of diseases in humans. However, the proposed strategy for the partner protein quest is not restricted to this scientific area but can be adopted in virtually every field of human biology where protein-protein interactions are assumed.


Useful keywords (using NLM MeSH Indexing)

Adenosine Triphosphate/chemistry

Amino Acid Sequence

Animals

Base Sequence

Caenorhabditis elegans/genetics*

DNA, Complementary/metabolism

Fluorescence Resonance Energy Transfer

Genome*

Humans

Ion Channels/genetics*

Ions/chemistry

Ions/metabolism

Light

Models, Genetic

Molecular Sequence Data

Oligonucleotide Array Sequence Analysis

Operon

Plasmids/metabolism

Protein Binding

Recombinant Fusion Proteins/metabolism

Reverse Transcriptase Polymerase Chain Reaction

Sequence Homology, Amino Acid

Species Specificity