PMU-Autor/inn/en
Dossena SilviaAbstract
How can a large number of different phenotypes be generated by a limited number of genotypes? Promiscuity between different, structurally related and/or unrelated proteins seems to provide a plausible explanation to this pertinent question. Strategies able to predict such functional interrelations between different proteins are important to restrict the number of putative candidate proteins, which can then be subjected to time-consuming functional tests. Here we describe the use of the operon structure of the nematode genome to identify partner proteins in human cells. In this work we focus on ion channels proteins, which build an interface between the cell and the outside world and are responsible for a growing number of diseases in humans. However, the proposed strategy for the partner protein quest is not restricted to this scientific area but can be adopted in virtually every field of human biology where protein-protein interactions are assumed.
Useful keywords (using NLM MeSH Indexing)
Adenosine Triphosphate/chemistry
Amino Acid Sequence
Animals
Base Sequence
Caenorhabditis elegans/genetics*
DNA, Complementary/metabolism
Fluorescence Resonance Energy Transfer
Genome*
Humans
Ion Channels/genetics*
Ions/chemistry
Ions/metabolism
Light
Models, Genetic
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Operon
Plasmids/metabolism
Protein Binding
Recombinant Fusion Proteins/metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Species Specificity