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Forschungsdatenbank PMU-SQQUID

Generation of human dendritic cells/Langerhans cells from circulating CD34+ hematopoietic progenitor cells.
Strunk, D; Rappersberger, K; Egger, C; Strobl, H; Kromer, E; Elbe, A; Maurer, D; Stingl, G
Blood. 1996; 87(4):1292-1302
Originalarbeiten (Zeitschrift)


Strunk Dirk


95%) from the peripheral blood of healthy adults. In vitro stimulation of these cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor (TNF)-alpha led to their vigorous proliferation and differentiation resulting in the emergence of CD45+/CD68+/CD3-/CD19-/CD56- leukocytes some of which (mean: 12%) express CD1a and exhibit anti-CD4 and anti-major histocompatibility complex (MHC) class II reactivity. These CD1a- leukocytes include (1) LC as evidenced by the presence of Birbeck granules (BG), (2) CD14+ monocytes, and (3) Birbeck granule-negative cells with a dendritic morphology. Addition of interleukin (IL)-4 to the cytokine cocktail interfered with the development of monocytes and led to a reduction in the overall yield but, on the other hand, resulted in an increased percentage of CD1a+ cells (mean: 24%) among all cells generated. In vitro generated CD1a+, but not CD1a- HPC-derived cells are potent stimulators of the primary mixed leukocyte reaction and, as such, promising candidates for vaccination purposes.

Useful keywords (using NLM MeSH Indexing)


Antigens, CD1/analysis

Antigens, CD34/analysis

Blood Cells/cytology

Cell Division

Cell Separation

Cells, Cultured

Dendritic Cells/cytology*

Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology

Hematopoietic Stem Cells/cytology*

Hematopoietic Stem Cells/immunology



Langerhans Cells/cytology*

Lymphocyte Culture Test, Mixed

Lymphocyte Depletion

Tumor Necrosis Factor-alpha/pharmacology