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Forschungsdatenbank PMU-SQQUID

Human dermis harbors distinct mesenchymal stromal cell subsets.
Vaculik, C; Schuster, C; Bauer, W; Iram, N; Pfisterer, K; Kramer, G; Reinisch, A; Strunk, D; Elbe-Burger, A
J INVEST DERMATOL. 2012; 132(3 Pt 1): 563-574.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Strunk Dirk

Abstract

Multipotent mesenchymal stromal cells (MSCs) are found in a variety of adult tissues including human dermis. These MSCs are morphologically similar to bone marrow-derived MSCs, but are of unclear phenotype. To shed light on the characteristics of human dermal MSCs, this study was designed to identify and isolate dermal MSCs by a specific marker expression profile, and subsequently rate their mesenchymal differentiation potential. Immunohistochemical staining showed that MSC markers CD73/CD90/CD105, as well as CD271 and SSEA-4, are expressed on dermal cells in situ. Flow cytometric analysis revealed a phenotype similar to bone marrow-derived MSCs. Human dermal cells isolated by plastic adherence had a lower differentiation capacity as compared with bone marrow-derived MSCs. To distinguish dermal MSCs from differentiated fibroblasts, we immunoselected CD271(+) and SSEA-4(+) cells from adherent dermal cells and investigated their mesenchymal differentiation capacity. This revealed that cells with increased adipogenic, osteogenic, and chondrogenic potential were enriched in the dermal CD271(+) population. The differentiation potential of dermal SSEA-4(+) cells, in contrast, appeared to be limited to adipogenesis. These results indicate that specific cell populations with variable mesenchymal differentiation potential can be isolated from human dermis. Moreover, we identified three different subsets of dermal mesenchymal progenitor cells.


Useful keywords (using NLM MeSH Indexing)

5"-Nucleotidase/analysis

5"-Nucleotidase/metabolism

Adipogenesis

Antigens, CD/analysis

Antigens, CD/metabolism

Antigens, Thy-1/analysis

Antigens, Thy-1/metabolism

Biological Markers/analysis

Biological Markers/metabolism

Cell Differentiation

Cell Separation*

Cells, Cultured

Chondrogenesis

Dermis/cytology*

Dermis/metabolism

GPI-Linked Proteins/analysis

GPI-Linked Proteins/metabolism

Humans

Male

Mesenchymal Stromal Cells/cytology*

Mesenchymal Stromal Cells/metabolism

Multipotent Stem Cells/cytology*

Multipotent Stem Cells/metabolism

Nerve Tissue Proteins/analysis

Nerve Tissue Proteins/metabolism

Osteogenesis

Receptors, Cell Surface/analysis

Receptors, Cell Surface/metabolism

Receptors, Nerve Growth Factor/analysis

Receptors, Nerve Growth Factor/metabolism

Stage-Specific Embryonic Antigens/analysis