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Forschungsdatenbank PMU-SQQUID

Isolation and large scale expansion of adult human endothelial colony forming progenitor cells.
Hofmann, NA; Reinisch, A; Strunk, D;
J Vis Exp. 2009;
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Strunk Dirk

Abstract


This paper introduces a novel recovery strategy for endothelial colony forming progenitor cells (ECFCs) from heparinized but otherwise unmanipulated adult human peripheral blood within a mean of 12 days. After large scale expansion >1x10(8) ECFCs can be obtained for further tests. Advantageously by using pHPL the contact of human cells with bovine serum antigens can be excluded. By flow cytometry and immunohistochemistry the isolated cells can be characterized as ECFC and their in vitro functionality to form vascular like structures can be tested in a matrigel assay. Further these cells can be subcutaneously injected in a mouse model to form functional, perfused vessels in vivo. After long term expansion and cryopreservation proliferation, function and genomic stability appear to be preserved. (3,4) This animal-protein free isolation and expansion method is easily applicable to generate a large quantity of ECFCs.


Useful keywords (using NLM MeSH Indexing)

Adult

Animals

Blood Cells/cytology*

Cell Separation/methods

Colony-Forming Units Assay

Endothelial Cells/cytology*

Flow Cytometry

Humans

Immunohistochemistry

Mice

Stem Cells/cytology*