Forschungsdatenbank PMU-SQQUID

PMU-Autor/inn/en

Abstract

This study aimed to investigate the expression of interleukin-6 (IL-6) in acute and chronic herpes simplex virus encephalitis. In the brain of 15 SJL mice infected with herpes simplex virus type 1, strain F, and 14 control animals we performed a sequential quantitative analysis of expression of IL-6 mRNA with reverse transcription real-time polymerase chain reaction. The viral burden peaked in the acute disease, and then returned to a low baseline value. At day 7 following infection, IL-6 expression was significantly (2.05-fold) increased as compared with the baseline expression in uninfected animals. Twenty-one days after infection the mRNA expression still was significantly (1.78-fold) upregulated. No significant differences of IL-6 mRNA expression between infected and control mice were found after 2 and 6 months. We observed a 2.5-fold increase of IL-6 mRNA expression in control mice with increasing age of animals. We have additionally studied the clinical evolution of HSVE in IL-6 deficient mice. In experimental herpes simplex virus encephalitis IL-6, as a potent mediator of neuronal injury, is upregulated in the acute but not in the chronic disease. IL-6 deficient mice presented early and severe clinical signs of HSVE as compared to the wild-type C57/bl6 mice.

Useful keywords (using NLM MeSH Indexing)

Animals

Brain/metabolism*

Brain/virology

Disease Models, Animal

Encephalitis, Herpes Simplex/metabolism*

Encephalitis, Herpes Simplex/virology

Female

Gene Expression Regulation

Herpesviridae Infections/metabolism

Interleukin-6/genetics

Interleukin-6/metabolism*

Mice

Mice, Inbred Strains

RNA, Messenger/biosynthesis

Reverse Transcriptase Polymerase Chain Reaction/methods

Simplexvirus*

Time Factors

Viral Load

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