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Forschungsdatenbank PMU-SQQUID

Increased presence of matrix metalloproteinases 2 and 9 in short- and long-term experimental herpes simplex virus encephalitis.
Martínez-Torres, FJ; Wagner, S; Haas, J; Kehm, R; Sellner, J; Hacke, W; Meyding-Lamadé, U;
Neurosci Lett. 2004; 368(3):274-278
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Sellner Johann

Abstract

Herpes simplex virus encephalitis (HSVE) causes elevated morbidity and mortality despite antiviral treatment. Virus-independent mechanisms may perpetuate brain damage. Matrix metalloproteinases (MMPs) target extracellular matrix components. This study describes the protein and mRNA expression of MMP2 and MMP9 in experimental HSVE in the short and long term. Ten SJL-NBOM mice were infected with neurovirulent HSV-1 and compared with nine controls. The presence of MMP2 and MMP9 in brain tissue was analyzed with sodium-dodecyl-sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gelatin zymography and mRNA expression of MMP2 and MMP9 with quantitative real-time PCR at days 7, 21 and 180 post-inoculation. Infected animals had a significantly elevated gelatinolytic activity of MMP2 at all time points, and of MMP9 at days 21 and 180. Increased presence of MMP2 and MMP9 in chronic HSVE may contribute to ongoing damage. Inhibition of MMP2 and MMP9 might be a suitable target for therapeutic intervention.


Useful keywords (using NLM MeSH Indexing)

Animals

Brain/enzymology

Brain/virology

Disease Models, Animal

Encephalitis, Herpes Simplex/enzymology*

Encephalitis, Herpes Simplex/genetics

Encephalitis, Herpes Simplex/pathology

Female

Herpesvirus 1, Human/enzymology*

Herpesvirus 1, Human/genetics

Matrix Metalloproteinase 2/biosynthesis*

Matrix Metalloproteinase 2/genetics

Matrix Metalloproteinase 9/biosynthesis*

Matrix Metalloproteinase 9/genetics

Mice

RNA, Messenger/biosynthesis

RNA, Messenger/genetics

Time Factors


Find related publications in this database (Keywords)

herpes simplex virus encephalitis
viral encephalitis
matrix metalloproteinases
gelatinases
SDS-PAGE zymography
HSV-1 quantitative PCR
quantitative real-time PCR
cranial magnetic resonance imaging