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Forschungsdatenbank PMU-SQQUID

Experimental herpes simplex virus encephalitis: a combination therapy of acyclovir and glucocorticoids reduces long-term magnetic resonance imaging abnormalities.
Meyding-Lamadé, UK; Oberlinner, C; Rau, PR; Seyfer, S; Heiland, S; Sellner, J; Wildemann, BT; Lamadé, WR;
J Neurovirol. 2003; 9(1):118-125
Übersichtsarbeiten

PMU-Autor/inn/en

Sellner Johann

Abstract

Despite early antiviral treatment, herpes simples virus encephalitis (HSVE) still remains a life-threatening sporadic disease with high mortality and morbidity. In patients and in experimental disease, chronic progressive magnetic resonance imaging (MRI) abnormalities have been found even after antiviral therapy. Secondary autoimmune-mediated and not directly virus-mediated mechanisms might play a key role for the outcome of disease. This study aimed to evaluate a possible beneficial effect of a therapy of acyclovir and corticosteroids versus acyclovir only. In a mouse model of HSVE (intranasal inoculation with 10(5) pfu [plaque-forming units] of HSV-1 strain F), a long-term MRI study was realized. Cranial MRI was performed serially at days 2, 7, 14, 21, 60, and 180 in different therapy groups: 1, saline; 2, acyclovir; 3, acyclovir, subsequently methylprednisolone; 4, sham-infected with saline. Brain viral load peaked at day 7 to decline thereafter to a low baseline value. Viral load in group 1 was significantly higher than in animals with antiviral therapy. In group 4, no viral DNA was detectable. Viral load did not differ significantly between acyclovir and acyclovir/corticosteroid-treated groups, suggesting that the use of corticosteroids in addition to acyclovir does not increase viral burden. MRI findings in untreated and acyclovir-treated animals revealed chronic progressive changes. In contrast, there was a significant reduction of the severity of long-term MRI abnormalities in acyclovir/corticosteroid-treated animals. With respect to abnormal MRI findings, this study demonstrates a clear beneficial effect of an acyclovir and corticosteroid therapy without influencing brain viral load.


Useful keywords (using NLM MeSH Indexing)

Acyclovir/pharmacology*

Animals

Antiviral Agents/pharmacology*

Drug Therapy, Combination

Encephalitis, Viral/drug therapy*

Encephalitis, Viral/pathology

Female

Glucocorticoids/pharmacology*

Herpes Simplex/drug therapy*

Herpes Simplex/pathology

Magnetic Resonance Imaging

Methylprednisolone/pharmacology*

Mice

Mice, Inbred Strains

Viral Load


Find related publications in this database (Keywords)

acyclovir
corticosteroids
experimental herpes simplex encephalitis
HSV-1 infection
magnetic resonance imaging